FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n01_vk9-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n01_vk9-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4801801
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61470.12801446790485488No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAAA676300.046.2708270
GTTGCCG57800.044.566753
GGTTGCC60550.043.40964552
CCGGGGT135250.041.94751757
TGCCGGG180000.041.7659455
CGGTTGC41200.041.6254951
TTGCCGG106950.041.06987854
AAAGATC188400.041.0611045
GTTGGCG93150.040.61657753
GCGGTTG65100.040.4831750
CGTGAGA18650.039.9779053
GAAGATC145850.039.9361465
GATGCCG19000.039.7887853
GGATGCC23600.039.74506852
GAAAAAA298050.039.1974470
GTGCCGG74800.038.92980254
GGGTGGA176000.038.8572866
GGTGGCG205750.038.852353
GTGAGAT51500.038.5385254
GCCGTGG27750.038.467856
Produced by FastQC (version 0.11.5)