FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n01_vk9-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n01_vk9-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5240217
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTG15100.025.0328658
GCGGGGT12600.024.16642257
GGGGGGA35200.022.9685267
TGGCGGG18150.022.75459355
GGGGGAA37950.021.1196868
ATGGGGG45650.020.01075247
CATGGGG47100.019.84056346
GGGTGGC15500.019.64496452
GCATGGG47800.019.62323245
ATCGGGG19250.019.27253347
GGCATGG54200.019.04962244
TAGGCAT92800.019.04614442
GGGGAAA45400.018.88747469
ACTTAGG96200.018.88234539
TTAGGCA97250.018.4985341
CTTAGGC97900.018.44720840
GGTGGCG14250.018.42086653
GGGAAAA51400.018.1807570
TGCGGGG12150.018.14796456
GGCGGGG28400.017.99277756
Produced by FastQC (version 0.11.5)