FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n01_vk9-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n01_vk9-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7441097
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGGG36700.030.23114655
TGCCGGG14850.027.81102455
ATCGGGG100400.027.39997947
CGGGGGT49900.026.7230449
TCGGGGG80600.026.44500548
TATCGGG112550.026.33900346
GGGTGGC33350.025.5018652
GTATCGG116950.025.49768845
CGGGGTG39150.025.29974458
CCGATGT164500.025.16982740
GCGGGGT28950.025.14642157
CGATGTA167500.024.65634241
TGTATCG141300.024.34853244
GGGGGGA73800.024.13919667
GGGGGAA76650.023.83525368
ACCGATG174250.023.72130439
GGTGGCG33350.023.50788753
CACCGAT177400.023.37901138
GATGTAT177550.023.10299942
ATGTATC173550.023.09097743
Produced by FastQC (version 0.11.5)