FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n01_vk9-21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n01_vk9-21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6357060
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG79050.12434993534747195No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT58300.042.1053121
GGGGAAA82700.040.20499470
GGGGGAA129050.028.58541770
GGGGTAA22550.028.24794270
CGGGGGT120200.027.07948751
GTTGGGG44700.026.93474656
GGGAAAA46300.026.9110770
GGGGGGA156350.026.3923469
GGTTGGG46200.026.0602455
GCCCACA27850.025.9017721
GGGGGTA27100.025.313369
TTGGGGG51050.025.02413457
AGTGGCG7800.024.67914239
CACTCTT157650.023.589461
TTGCGGG17300.023.2655757
GGCGGTA7750.023.03193542
GGGTTGG55400.022.55383554
GAGATCG152100.022.4605356
CGCGAGA7550.022.253273
GGGATTC23000.022.2170832
Produced by FastQC (version 0.11.5)