FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n01_vk9-20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n01_vk9-20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5117438
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65740.12846271904026976No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAAA122950.041.93085570
GGGAAAA61100.040.6702370
ACACTCT34550.037.402161
TTGCGGG39950.036.8828857
GGGTGGA38000.036.10455368
GGTGGCG69900.035.44994755
GTTGCGG35500.035.3936756
GCGTGGA13200.034.99931368
GGCGTGA6300.034.9993167
GTGGCGG82800.034.9570456
GTGGAAA54050.034.5136670
TGGCGGC20400.034.3130557
GGTTGCG31450.034.1646755
GGGTGGC76550.033.559154
CGGGGGT129700.032.9754551
TGGCGGG91050.032.8659157
GGGCGTG16950.032.21175466
GCGGCGT14600.032.12265459
CGCGGGT26850.031.80570651
GGGGGAA147650.031.36071269
Produced by FastQC (version 0.11.5)