FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n01_vk9-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n01_vk9-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6633136
Sequences flagged as poor quality0
Sequence length76
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70000.105530777599012No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT39050.042.055511
GCCGTGG81200.036.68048556
GCGTGGA92300.036.6366
CGCTTGC7200.035.97168723
CCGTGGT81750.035.62026257
GGTCGGC10150.034.82706561
GGGTGGA64050.034.69893366
CGTGGTG75050.034.6963558
TGCCGTG116050.034.5320155
GGAAAAA275050.034.40777670
CGTGGAA99700.034.05164767
GTGGAAA169400.033.4292568
GCGCTTG7500.033.1328422
TCGGCGT66600.033.10761363
GAGTCAC7150.032.796719
GTCGGCG44900.032.66098462
GCCGTGT33850.032.05269656
ACGAGTG7150.031.81770538
TGCCGGG46800.031.40978655
CGTGGTC16900.031.27172358
Produced by FastQC (version 0.11.5)