FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n01_vk9-19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n01_vk9-19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3683644
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47720.12954563470302777No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGAA42250.042.16503568
GGCGTGA27550.039.76345467
CGTGAAA47100.038.4176769
ACACTCT26200.037.0208361
TGCGTGA18700.036.4967767
CTGCGTG18150.034.32454766
GGGCGTG23150.033.71438266
GTGAAAA63550.032.71389870
TGGGCGT24000.032.22867265
GTGGGCG21850.031.71576164
TGGCGGG10550.031.18435757
TCGGCGT31350.030.81292565
GGGTGGA23700.030.27379868
GGGGGGA23050.029.60909368
GGCGGGA4750.029.47323267
GGGGGAA23850.029.20290669
GGGGAAA30550.028.98482570
GTGTGGC12250.028.2852854
CCGTCGT53350.028.01269159
GCCGTCG54300.027.84487758
Produced by FastQC (version 0.11.5)