FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n01_vk9-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n01_vk9-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3300337
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44190.133895417346774No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT28150.043.6635931
GGAAAAA150150.038.15783770
GGTCGGC6050.037.02414761
GCCGTGG36700.036.1437856
GGGTGGA38200.035.91560466
GCGTGGA42750.035.6134166
CCGTGGT37250.035.422257
CGTGGTG37000.034.62101758
GTGGAAA85850.034.2451868
TGCCGTG53600.033.6934455
CGTGGAA45850.033.2818667
TGGTCGG6950.033.23682860
CCGGGGT39800.032.62505757
GCCGTGT15500.032.51555656
CGTGGTC8600.031.7436358
TGGAAAA103850.031.71345369
CGGGGAA12700.031.41677367
GTGGTCG7250.031.37876159
GGTGGAA45000.031.34389567
TCGGCGT24150.030.86902263
Produced by FastQC (version 0.11.5)