FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n01_vk9-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n01_vk9-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4737735
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55710.11758783469316035No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT48000.041.2147521
GGGGAAA79400.038.3055270
GGGAAAA48900.032.70916470
GGGGGAA84200.031.54944669
GGGTGGA32200.031.19522368
GCGTGAA14500.031.137568
GGGGGGA85200.030.76835668
GGCGTGA10500.030.33291867
TTGCGGG33100.029.81832357
GTTGCGG29150.028.4558756
TGCGGGG35950.027.84384758
GCGTGGA17500.027.79961868
GTGGGCG25150.027.55429364
GTGGCGG49300.027.54526156
GGTTGCG25350.027.33690355
GCGGGGA14250.027.26278368
TGGCGGG59900.027.22834457
GCGGGGT49800.027.1281459
GGTGGCG39700.026.9769655
GTGGAAA56900.026.94163370
Produced by FastQC (version 0.11.5)