HKT2VAFX2_n01_vk9-16.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HKT2VAFX2_n01_vk9-16.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5382493
Sequences flagged as poor quality	0
Sequence length	76
%GC	38

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG	9273	0.17228076283610588	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	6864	0.12752455042672606	No Hit
ACACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCA	5985	0.11119382784148535	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CACTCTTTCCCTACACGACGCTAGATCGGAAGAGCACACGTCTGAACTCC	5929	0.1101534177564188	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC	5607	0.10417105976728627	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
ACACTCT	10025	50.819122	1
GCGTGAA	13100	42.07966	68
TGCGTGA	6095	40.540947	67
GGCGTGA	7535	40.22514	67
GTGAAAA	15850	38.753487	70
ACGAGTG	870	38.217937	38
CTGCGTG	6515	37.712524	66
GGGCGTG	5060	37.420506	66
CGAGTGA	905	36.35316	39
CGGAGTC	940	35.744255	7
TGGGCGT	5335	35.294804	65
ACGGAGT	935	35.18936	6
CACGAGT	935	35.18675	37
GTGGGCG	4905	35.035263	64
GAGTCAC	975	34.820103	9
GTCACTC	965	34.81824	11
GTGAGGT	945	34.814404	42
GGCGGGA	1215	34.27943	67
GGGTGGA	4800	33.90585	68
GTGCGTG	1405	33.629498	66