FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n01_vk9-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n01_vk9-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5515969
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG66230.12006956529306093Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65300.1183835514666598No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT74850.046.032251
GCGTGAA93700.041.94712468
GGCGTGA67200.040.5202767
CGTGAAA100700.039.48306769
GGGAAAA97000.038.7159670
GGGGAAA151450.038.0846570
GTGAAAA145050.037.25560470
TGCGTGA32600.037.25408667
GTGGCGG66700.035.99650256
GGGCGTG76700.035.82088566
CGTATGG31250.035.3915153
GCGGGGT84900.034.46359659
GGTGGCG44200.034.3660455
TGGCGGG96000.034.2703657
CTGCGTG30500.034.08149766
TGGGCGT84200.034.00190465
GGGTGGA93050.033.55138868
TTGCGGG45850.033.51098657
GTGGGCG92050.033.2314864
GTATGGC35050.033.05232254
Produced by FastQC (version 0.11.5)