FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n01_vk9-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n01_vk9-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3917148
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG91970.2347881673094813Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC65430.16703479163922322Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64470.1645840289925221No Hit
CACTCTTTCCCTACACGACGCTAGATCGGAAGAGCACACGTCTGAACTCC63110.16111211524302885Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCA61940.15812524826736188Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC56360.1438801903834116Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTC47850.12215520067150898TruSeq Adapter, Index 16 (100% over 38bp)
CTCTTTCCCTACACGACGCTAGATCGGAAGAGCACACGTCTGAACTCCAG46780.11942362147154002Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGT46710.11924492002855139TruSeq Adapter, Index 16 (100% over 37bp)
CACTCTTTCCCTACACGACAGATCGGAAGAGCACACGTCTGAACTCCAGT46360.11835141281360828Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCC44840.11447103862299815TruSeq Adapter, Index 16 (100% over 40bp)
ACACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGT43700.11156075798004059Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCC40070.10229381172220198TruSeq Adapter, Index 16 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT109550.052.929121
GGGAAAA66800.043.69717470
ACGAGTG5700.042.98203338
GCGTGAA99650.042.18222468
GTGAAAA122550.041.89677470
GGCGTGA61400.041.49796367
GTGAGGT6000.041.41625642
CGTGAAA103200.041.23990269
GGGGAAA104850.040.35725870
TGGAAAA91650.038.8375970
TGCGTGA42900.038.58936367
CGAGTGA6550.038.47290439
CACGAGT6500.038.23039237
ACGGAGT6550.037.4061246
CGGAGTC6400.037.1871347
CTGCGTG43600.037.0065266
GGGTGGA70250.036.76832268
GCGTCGT10300.036.6986759
CGTATGG25800.035.813653
GGGCGTG61550.035.76731566
Produced by FastQC (version 0.11.5)