FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n01_vk9-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n01_vk9-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6575232
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAAA48200.029.04514170
GCGGGGT26150.027.97274859
TGCGGGG22100.027.87282258
GGGGGGA44150.027.66656568
TGGCGGG27250.025.81606957
GGGGGAA47550.025.76191169
CGGGGTG31850.024.17540660
TTGCGGG25700.022.47042857
GGGTGGA18950.020.31627368
GCGGGGA8550.020.05813268
CGGGGGT27250.019.90791351
CGCGGGT15300.019.44410751
GGTTGCG19000.018.97335655
GGGTGGC24050.018.77306654
GTTGCGG22650.018.54272756
GGCGGGG42300.018.53395858
GGTGGCG22200.018.28797155
GTGGCGG27650.018.10095456
GTGGGGG54000.017.8885864
ACACTCT39400.017.864531
Produced by FastQC (version 0.11.5)