FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n01_vk9-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n01_vk9-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6417035
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAAA33700.025.6524670
CGGGGGT29550.025.10963251
ACACTCT34850.024.8164081
GGGGGGA49500.023.96936269
GGGGGAA40250.023.91270870
GCCCACA19600.019.2935621
TCGGGGG112000.018.5622450
CACTCTT73150.018.2370831
ATCGGGG114550.018.11847149
AATCGGG118700.017.4260448
GAATCGG127500.016.63506147
GGAATCG134600.016.0696146
GGGGGAG12200.015.49158670
GGGGGTA11550.015.454329569
CGGAATC144500.015.38040545
GGGGTTG23300.015.32167353
AGGGGGG12350.015.3098711
TCGGAAT183700.014.89908344
GGGTGGG47050.014.50564254
GTTTCGG205050.014.32069741
Produced by FastQC (version 0.11.5)