FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n01_vk9-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n01_vk9-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2739852
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41170.15026359088009134No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT41800.046.4923931
GCCCACA21150.040.0654681
GGGTGGA13550.030.22077866
GGAAAAA48550.028.33103270
GCGTGGA11400.027.63101266
GTGGAAA27900.026.34354268
CACTCTT99850.024.1621931
GGTGGAA18200.023.84566367
GCCGTGG14750.023.72832756
AGTGGCG3850.023.63587639
GCACCCA11500.022.227481
CGTGGTG15350.022.11680858
TGGAAAA37900.021.9784469
CGTGGAA14250.021.859267
CCGTGGT15950.021.7236957
CGGGGAA4850.021.6490467
GGCGGTA4050.021.60449242
ACTCTTT114550.021.1176262
GGGGAAA21300.020.53948268
GCGGGGA5350.020.27995566
Produced by FastQC (version 0.11.5)