HKT2VAFX2_n01_vk9-10.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HKT2VAFX2_n01_vk9-10.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4111007
Sequences flagged as poor quality	0
Sequence length	76
%GC	47

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG	8927	0.21714874238842213	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	7042	0.17129622985317222	No Hit
ACACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCA	6144	0.1494524334305439	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CACTCTTTCCCTACACGACGCTAGATCGGAAGAGCACACGTCTGAACTCC	5290	0.1286789343827437	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC	5200	0.12648968975241345	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC	5178	0.1259545410649994	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT	4164	0.10128905156327878	TruSeq Adapter, Index 10 (100% over 38bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
ACACTCT	8975	52.15693	1
GGAAAAA	24875	49.822407	70
TGCCGGG	4300	40.697098	55
TTGCCGG	2425	39.834484	54
AAAGATC	11485	39.316364	5
GGGAAAA	45765	38.857746	70
GTTGCCG	2080	38.0283	53
CCGGGGT	2305	37.80857	57
CGTGAGA	1250	37.524033	3
GGTTGCC	2190	37.0771	52
GCGTGAG	570	36.847404	2
GTTGGCG	5405	36.780235	53
TTGGCGG	5870	35.893867	54
GTGAGAT	3200	35.769466	4
GGTTGGC	5770	35.727394	52
GTGCCGG	2715	35.5796	54
CGCGTGA	155	33.882435	1
GCGAGAT	4205	33.879974	4
TGGCGGG	23110	33.74249	55
GTGGAAA	4975	32.994507	68