FastQCFastQC Report
Thu 17 Dec 2020
HKT2VAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKT2VAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10500041
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3217103.0638927981328834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGATC54000.031.5927855
CACTCTT76900.031.3337211
GGAGATC22300.031.1968025
AAGATCG185850.030.6422316
GAGATCG129750.029.3684466
GTGAGAT15550.029.2263664
GCGAGAT17750.028.5582474
GCGCTAG2950.028.4423542
GAAGATC50000.027.8974655
GGAAAAA138450.027.86605870
TGAGATC38650.027.677945
GCTTGAA38100.027.34435316
GTCACCT62700.026.88332736
CGCGAGA8500.025.910963
CGAGATC61000.025.3311165
GCCCACA13650.025.1080231
CCTTGTA53200.025.03406540
ACCTTGT54000.024.98794439
CACACGT456650.024.8062719
CGCGAAG5800.024.7133852
Produced by FastQC (version 0.11.5)