FastQCFastQC Report
Wed 29 Jul 2020
HKNWNDRXX_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKNWNDRXX_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1928936337
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT57270950.067.34151
AGCAGTG59287500.064.981692
GCAGTGG66600600.057.8305053
CAGTGGT70478500.054.6351854
AGTGGTA73058700.052.6909185
TGGTATC77180750.049.8450137
GTGGTAT77407350.049.721146
GGTATCA78231850.049.2126668
GTATCAA85599750.045.102769
CATGGGG48408000.028.40235724-25
AGAGTAC77648300.027.80703418-19
GCAGAGT78845300.027.51902816-17
TACATGG77571350.027.4763422-23
CAACGCA80337200.027.2103612-13
ACGCAGA81947600.026.62701214-15
ATCAACG82222750.026.59128210-11
AGTACAT84640750.025.3665720-21
ACATGGG75622800.024.73891624-25
CAGAGTA78521850.024.42215318-19
TCAACGC79678100.024.25908512-13
Produced by FastQC (version 0.11.5)