FastQCFastQC Report
Wed 29 Jul 2020
HKNWNDRXX_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKNWNDRXX_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1928936337
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTGCGG566020202.934364339262276No Hit
TCGCCAGC546871842.835095329535492No Hit
TAGGACGT532459012.7603762746681046No Hit
GGCTGTTG515600922.672980492460908No Hit
TATGAGCT502029342.602622649437911No Hit
CCGATAGC495744232.570039355321658No Hit
ACACCTTC478600582.481163171742355No Hit
GCGGATAG474727882.4610863038555535No Hit
GGGGGGGG472951422.451876772333311No Hit
CCTTGTAG471330172.4434718811562313No Hit
CTCGAAAT464058532.405774213998852No Hit
AGCAGAGC449954522.3326561450949534No Hit
CATCTCCA447510332.3199849648537154No Hit
ATACCCAA443767872.3005832877313876No Hit
GGAATGTC438337032.272428703799155No Hit
GTCAGCTA433219202.2458968276463134No Hit
TTATCGTT433175052.245667945027675No Hit
ATCCCACA431026592.234529889515996No Hit
CGATAGCT413978782.1461505600741866No Hit
TTGCCGGA405371982.101531150740098No Hit
AGGCCCGA403293082.090753708477627No Hit
GGTATGCA380648741.973360824297645No Hit
GCCGAGAT375905481.9487707955392206No Hit
CCAAGATG368268231.9091777314577074No Hit
GCCATTCC366680921.9009487921736425No Hit
ATGCCTTA343263381.7795474812500254No Hit
TGGAGGCT337505401.7496969367320285No Hit
AGTTGCAG334684671.7350736962139564No Hit
GTTTATCT319139281.6544832189555048No Hit
CGTATCGG318441101.650863711216406No Hit
TAATGACC315743111.6368767799307622No Hit
GTACGCGG312120331.6180955483757884No Hit
CAAGAATT308872091.601256008689104No Hit
CACGTCGG305837531.585524229771405No Hit
TACGTGAC297591461.5427749184445998RNA PCR Primer, Index 17 (100% over 8bp)
AGCACACT292618501.516994077964741No Hit
TGCGGCTT286080471.4830995949038415No Hit
GAATCCGC285389731.4795186576445316No Hit
AATGTTAG283367771.469036403973347No Hit
ACGACATT278390541.443233426941244No Hit
CCTTTGTC264839651.3729828451046489No Hit
TAGGATAA253244311.3128702339334903No Hit
CCACTACA238697381.2374559772731368No Hit
TGTCAGAC234393001.2151411920859054No Hit
GTGCCCGT231922331.2023327341155272No Hit
GTATGTCA230482711.194869449960494No Hit
ATGAAGAC225871101.1709619216945677No Hit
CCCGATTA225013531.1665161036364466No Hit
TCAGGGTG223774621.1600933411209828No Hit
ATTCTAAG202435481.0494668803577003No Hit
CATATAAC192829810.999669124901762No Hit
AGCTATCA180841820.9375209359229361No Hit
GATTCTGG167861900.8702303791999124No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)