FastQCFastQC Report
Wed 29 Jul 2020
HKNWNDRXX_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKNWNDRXX_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1928936337
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGAA2936900.016.0279751
GGGACCT3009600.015.832351
ATCGGCG2548450.015.7427231
CCGGACA3018500.015.4383271
GGGCTAC2743300.015.3877261
CGGAACC3124750.015.2577161
CCCGGAA2417050.015.0910461
TGGGCTG2680950.015.085321
GTGCTGG2742950.014.9476931
GGCACGT3248550.014.9383791
CTGGACG2123200.014.8961041
CTGTGGG3080800.014.8222381
CGGAGAA3347350.014.6558751
AACGGGA2650700.014.6469451
AGGCTGC3710900.014.6016041
GGGATCC3605700.014.5760481
TACCGGG2969400.014.4842951
AGGGAAG1997650.014.4691873
TTGATGG3364000.014.4580811
AATGGCT3603700.014.4055441