FastQCFastQC Report
Fri 22 Apr 2022
HKNNLAFX3_n02_10x_Pura_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKNNLAFX3_n02_10x_Pura_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91958370
Sequences flagged as poor quality0
Sequence length90
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA16086451.7493187406431847Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCAGATGTGTATAAGAGAC11437301.2437475783879162Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5796360.630324352204155Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA3980370.43284477530430343Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2314410.2516801896336353No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA1968520.21406643027709168Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG1955900.21269407015370106Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGTCAGATGTGTATAAGAGACAG1765310.19196838743444453Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA1540740.16754755439880026Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACAACCTCAACTCATATGGG1400740.15232327410762064Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGTCAGATGTGTATAAGAGACA1236630.13447715526058152Clontech SMARTer II A Oligonucleotide (100% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)