FastQCFastQC Report
Wed 5 Oct 2016
HKNHCBGXY_n01_timecourse_shoot_45k3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKNHCBGXY_n01_timecourse_shoot_45k3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19314000
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3752671.9429791860826342No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1502220.7777881329605467No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212680.11011701356528944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG22900.011.77340333
GCGGTAT17700.010.6788491
GCGGAAT25600.010.6649441
ATTTTTT212450.010.149111
CTTCCGA55150.09.4568111
CGGGCAT42950.09.4536331
CCGGGAT65650.09.4371851
TTCCGAT54050.09.2590322
GCCGAAT32950.09.2420391
TCGTATG29300.08.95934545
CGGCTAT24450.08.875991
GGGAAAT73200.08.846361
CGGCAAT60000.08.6923861
TATGCCG31500.08.66704748
CGTATGC32700.08.56300146
TCCCTAC38300.08.4072679
GCGGGAT48500.08.3718261
CTCGTAT29300.08.36209944
GGCGAAT28350.08.2722891
GCCAGAT87250.08.0235871
Produced by FastQC (version 0.11.2)