FastQCFastQC Report
Wed 5 Oct 2016
HKNHCBGXY_n01_timecourse_shoot_45k2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKNHCBGXY_n01_timecourse_shoot_45k2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18643305
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2764491.4828325771637594No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1219950.6543635905758126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA51350.011.1114341
TTCCGAT53300.010.7025632
CCGGGAT65500.010.2608391
GCCGGAT62150.09.6311451
CGGGCAT43350.09.3668131
ATTTTTT193650.08.89345551
GCCGAAT34650.08.3848971
GCGAAAT39450.08.3407241
CGCGAAT19500.08.2574541
GGGAAAT68800.08.242321
TCCCTAC38100.08.1758569
CGGGGAT63800.08.1201481
GGAAAAT103850.08.0896121
TCGTATG27350.08.06252745
GAAAAAT137650.08.0104571
GGCGAAT31150.07.97853661
GGCAAAT79300.07.90137671
GGGAGAT90500.07.89051
CTCGTAT28000.07.750376744
TTCCCTA39900.07.719288
Produced by FastQC (version 0.11.2)