FastQCFastQC Report
Wed 5 Oct 2016
HKNHCBGXY_n01_timecourse_shoot_45k1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKNHCBGXY_n01_timecourse_shoot_45k1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17426681
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2006641.1514757170341272No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA536180.3076776352307132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA49250.012.5798941
TTCCGAT49200.012.3059092
GGCGAAT27250.09.5055051
GGGAAAT70250.09.1681441
CCGGGAT50900.09.1462661
CGGGGAT52150.09.0612771
GCCGAAT28700.08.2934841
GGGAAAC61550.08.2461251
GGAAAAT105800.08.1387821
GGGGAAT47800.08.12841
CCGATCT73450.08.1002524
CGGCAAT53500.08.0475051
GGGATAT53250.07.8880851
TCCGATC74100.07.8873023
TATGCCG27800.07.80585948
CGGGTAT16700.07.7552391
GGGAGAT87150.07.5910941
CGCGAAT17650.07.53613761
CGGGAAT32650.07.5045531
GCGGGAT41250.07.4673871
Produced by FastQC (version 0.11.2)