FastQCFastQC Report
Wed 5 Oct 2016
HKNHCBGXY_n01_timecourse_shoot_30n3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKNHCBGXY_n01_timecourse_shoot_30n3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18872908
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1021980.5415063751701645No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA384380.20366760649710153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT27950.09.8928344
GGGAAAT76700.09.8119731
TTCCGAT54550.09.687572
CTTCCGA54200.09.5581891
GCCGAAT34800.09.4550071
CGTATGC33150.09.18567346
GCGGAAT28300.09.1528961
GTCGAAT35000.09.1009471
TCGTATG31850.08.90124645
TATGCCG31700.08.83295448
GGGAGAT93800.08.5456651
GGAAAAT116700.08.4584631
CCGGGAT66150.08.3606581
GGCGAAT30850.08.2828711
CGGGCAT42900.08.1593571
GCCAGAT90700.08.0658881
GCCGGAT61350.07.87367961
GGGGAAT53100.07.8445071
GTCCGCA36700.07.82290534
GGGATAT55450.07.7645591
Produced by FastQC (version 0.11.2)