FastQCFastQC Report
Wed 5 Oct 2016
HKNHCBGXY_n01_timecourse_shoot_30n2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKNHCBGXY_n01_timecourse_shoot_30n2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15012235
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAAAT52600.010.0488021
CGCGAAT15250.09.8701031
GCCGAAT26200.09.4859351
CTCGAAT28250.09.1693031
GGAAAAT77350.09.141431
CCGGGAT56900.09.0433261
CGAATCT27850.08.6707053
GAAAAAT100350.08.5112931
GCGAAAT28950.08.2221131
GGCGAAT23850.08.2190711
GGGACAT41500.08.181761
CGGGGAT52400.08.1498881
CGCGTAT10951.4551915E-117.9918811
GCCAGAT70100.07.9896011
GCGGAAT21250.07.9068841
TCGAATT34450.07.82225562
CGGCAAT50150.07.8175411
CGGGCAT34800.07.74523451
GGGAAAC48450.07.7305981
GGCAAAT61700.07.7157271
Produced by FastQC (version 0.11.2)