FastQCFastQC Report
Wed 5 Oct 2016
HKNHCBGXY_n01_timecourse_shoot_30n1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKNHCBGXY_n01_timecourse_shoot_30n1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16990002
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGAAT24050.010.4795651
GGGAAAT64600.09.64524651
GGAAAAT95400.09.5033611
GGCGAAT28800.09.1157961
CCGGGAT58900.09.033461
GCCGAAT31050.08.90617751
CGGCAAT53200.08.8827611
CGGGCAT41100.08.7724561
GCCGGAT56700.08.5813881
CGCGAAT18150.08.4859781
GCCAAAT70200.08.3273191
CGGGACT20600.08.326351
GCCAGAT79150.08.2702091
GCGAAAT34750.08.1593591
CCGGAAT36650.08.1184061
CGGGGAT61150.08.0713921
AATCGAA54950.08.0248445
GGGAGAT81800.07.95949361
GGAAAAC65600.07.95075271
GGGAAAC55150.07.68007851
Produced by FastQC (version 0.11.2)