FastQCFastQC Report
Wed 5 Oct 2016
HKNHCBGXY_n01_timecourse_shoot_30k3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKNHCBGXY_n01_timecourse_shoot_30k3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17873606
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201440.11270249551209754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAAAT66100.010.1144231
GGAAAAT98400.09.7824311
CCGGGAT65400.08.4564581
GGGGAAT49000.08.429371
GGCGAAT29800.08.10479451
CGGGCAT42800.08.0965611
GCGAAAT35050.08.0892111
GGAAAAC70250.08.0719391
GAAAAAT124800.07.8252611
CGGGACT20150.07.81711771
GCCAAAT76450.07.78360181
CGCGAAT21150.07.7785151
GCCGAAT31150.07.7535441
CTCGGAT39750.07.74916121
CGGGGAT63100.07.6552421
GGCAAAT78050.07.62404061
CGGAAAT33600.07.6048871
GTCGAAT34950.07.51144171
GCCGATT40700.07.4822811
CGGCAAT53850.07.47517441
Produced by FastQC (version 0.11.2)