FastQCFastQC Report
Wed 5 Oct 2016
HKNHCBGXY_n01_timecourse_shoot_30k2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKNHCBGXY_n01_timecourse_shoot_30k2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19678924
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAAT30900.010.0820161
GCGGAAT27050.09.5759151
CGGGTAT20050.09.4274721
CGGGAAT32150.09.2545191
GGAAAAC81500.09.1482471
GGAAAAT108600.08.9282391
GGGAAAT77450.08.7227161
CGCGAAT21050.08.6470341
GCCGAAT34450.08.6366551
GCGAAAT40900.08.47281551
CCGGGAT61900.08.3127081
CGGGGAT64450.08.3096821
GGGAGAT96300.08.2511681
CTCGGAT46100.08.1245391
GCCAGAT94250.08.0221031
GGGACAT56000.08.0008811
GGGATAT50800.07.9241011
GGAAGAT174500.07.883391
GCCAAAT84450.07.87534951
CGGGCAT44200.07.84023051
Produced by FastQC (version 0.11.2)