FastQCFastQC Report
Wed 5 Oct 2016
HKNHCBGXY_n01_timecourse_shoot_30k1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKNHCBGXY_n01_timecourse_shoot_30k1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20884014
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230690.11046248101538333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT25050.010.4806121
GGGAAAT79700.09.17954351
GCCAAAT88500.08.7414231
GCCAGAT97750.08.5946381
CGGCAAT66200.08.5662381
GCCGAAT41900.08.5215641
GCCGGAT69400.08.4234511
GGCAAAT93650.08.372851
CGGGGAT71550.08.3660341
GGGACAT58300.08.346021
CGGGCAT48950.08.1523961
GGAAAAT115600.08.0548411
CCGGGAT76000.07.9682991
GGGATAT57300.07.88076261
GGGAGAT103350.07.82410431
CTCGGAT50750.07.65631961
CCGGAAT48750.07.61139731
GGGGAAT58300.07.56545641
GGAAGAT180950.07.56399541
GGCGAAT32550.07.52800941
Produced by FastQC (version 0.11.2)