FastQCFastQC Report
Wed 5 Oct 2016
HKNHCBGXY_n01_timecourse_shoot_20n3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKNHCBGXY_n01_timecourse_shoot_20n3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21146312
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243760.11527305565150084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGAAT41200.09.0056991
GCCGGAT74100.08.8334881
GGGAGAT107600.08.718291
CCGGGAT76200.08.6819181
GGAAAAT110150.08.675351
CGCGAAT23100.08.6371751
GGCGAAT33350.08.6065051
GGGAAAT79150.08.3583341
CCGGAAT47900.08.3306371
GCGGAAT29150.08.2855121
CGGGTAT21150.08.2750091
CCCGTAT17150.07.9599311
GCCAAAT88150.07.9020471
GGGACAT56350.07.888941
GAAAAAT141650.07.78400041
CGGCAAT62800.07.7475431
CGGGAAT39500.07.70958471
GGGATAT57100.07.6627161
GCGAAAT48000.07.5840461
CTCGGAT49050.07.4216961
Produced by FastQC (version 0.11.2)