FastQCFastQC Report
Wed 5 Oct 2016
HKNHCBGXY_n01_timecourse_shoot_20n2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKNHCBGXY_n01_timecourse_shoot_20n2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18093853
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303040.1674822935722977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAAT95200.08.8982531
CGCGAAT21800.08.831461
CTCGGAT46650.08.7042771
GGGAAAT67950.08.6030681
GTCGATT40150.08.4569261
GTCGAAT35150.08.3652761
CGCAAAT35900.08.1905151
GAAAAAT119350.07.8602931
GGCGAAT29400.07.85819771
GCGAAAT39150.07.77882151
CGGGCAT42500.07.6598521
GGGAAAC65500.07.64224771
CCGGGAT64850.07.61089041
CTCGAAT38950.07.5491521
GGAAAAC67400.07.47874831
CGAATCT35150.07.46738673
GCCAGAT83800.07.4353661
GGAAATT81650.07.3310521
CCCGACT29250.07.1804511
GCCGAAT32300.07.15266281
Produced by FastQC (version 0.11.2)