FastQCFastQC Report
Wed 5 Oct 2016
HKNHCBGXY_n01_timecourse_shoot_20n1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKNHCBGXY_n01_timecourse_shoot_20n1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19908158
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273630.13744616654137465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAAAT73250.010.2745911
GGAAAAT108850.09.6156031
GCGGAAT29300.09.557761
GGCAAAT85400.08.9767681
CCCGTAT14600.08.8712041
CGCGAAT23250.08.58194351
GGGAGAT97200.08.5712561
CCGGGAT73150.08.5658881
GCCGAAT35800.08.5068731
GCGAAAT42700.08.361921
CGGCAAT62950.08.2299991
GCCAAAT81550.08.1557411
CTCGGAT45950.08.1513961
CGGGACT26050.08.062641
CGGGCAT46900.07.91164451
CTCGAAT41200.07.9016811
GCCGGAT71250.07.8117081
GGAAAAC76550.07.77387331
GGGATAT48850.07.66748481
GCCAGAT93150.07.6662161
Produced by FastQC (version 0.11.2)