FastQCFastQC Report
Wed 5 Oct 2016
HKNHCBGXY_n01_timecourse_shoot_20k3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKNHCBGXY_n01_timecourse_shoot_20k3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17854462
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198760.11132231259614543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAT27200.010.1667881
CCGGGAT58750.09.7715071
CGGGCAT42650.09.5206051
CTCGAAT36200.09.2829981
CGGACAT46350.09.062691
GCCGAAT29850.08.9124041
GGGACAT52250.08.8432771
GGGAAAT68000.08.751161
GGAAAAT106250.08.6317321
CTCGGAT43450.08.5396811
CCCGAAT23050.08.504381
CGCAAAT35050.08.4889991
GGAAAAC75850.08.4454171
CGCGAAT19500.08.4370161
CGGCAAT52100.08.0624891
CGCGTAT12550.07.80979971
GCCGGAT56150.07.7926621
CGGAAAT34250.07.7674531
GCGGATT33550.07.72084431
GCCAAAT81450.07.6069011
Produced by FastQC (version 0.11.2)