FastQCFastQC Report
Wed 5 Oct 2016
HKNHCBGXY_n01_timecourse_shoot_20k2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKNHCBGXY_n01_timecourse_shoot_20k2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15899905
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAAT29250.010.0526661
GCCGAAT28850.09.9493781
CGCAAAT32250.09.1175351
GGAAAAT97450.09.0520421
CGGGCAT37200.09.0335031
GGCGAAT25500.08.7855241
CCGGAAT30400.08.4057631
CGGGAAT25950.08.3633871
GGAAAAC67550.08.3431181
CCGGGAT52450.08.3424261
CTCGGAT36250.08.3045921
CGGGGAT51950.08.2879561
CGGAAAC32850.08.2050881
CTTAAAT45300.08.1137011
CGGAGAT102700.08.0780361
CGACAAT49050.08.0643111
CGCGAAT18500.07.947041
CCGGTAT19650.07.838231
GGGAAAT61000.07.80435371
GCGGAAT20650.07.79768471
Produced by FastQC (version 0.11.2)