FastQCFastQC Report
Wed 5 Oct 2016
HKNHCBGXY_n01_timecourse_shoot_20k1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKNHCBGXY_n01_timecourse_shoot_20k1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16267368
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAT24600.011.2408891
GCCGAAT29650.010.624941
CCGGGAT60750.010.1408651
CGGCAAT52850.09.6035471
GGAAAAT94050.09.6021741
GGGAAAT58150.09.5709721
CGGGACT18850.09.2846881
GCCGGAT58500.09.1547021
CGGAAAT30100.08.9543261
GTCGAAT30350.08.8805671
CGGGCAT40650.08.8692191
GGGACAT44250.08.8595861
GCCAGAT75850.08.7681241
CGGAGAT113750.08.7392791
CGGACAT46550.08.7226211
CGGGGAT59500.08.7066971
GGGAGAT85550.08.4695241
GCCAAAT70200.08.4267141
GGGATAT45100.08.3821581
GGGGAAT45500.08.308471
Produced by FastQC (version 0.11.2)