FastQCFastQC Report
Wed 5 Oct 2016
HKNHCBGXY_n01_timecourse_shoot_15n3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKNHCBGXY_n01_timecourse_shoot_15n3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18084699
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGAAT32200.010.5447791
GGCGAAT30800.09.43298151
CCGGGAT64950.09.3237051
CCGGAAT36800.09.3218021
GGAAAAT102900.09.2528411
GGGAAAT66750.09.072281
CGGGAAT28600.09.0570611
GGGACAT51550.08.9632791
CGGCAAT56950.08.9124241
CGGGCAT45350.08.8765091
CGGGGAT63500.08.5994861
CGGGACT22650.08.34541
CTCGGAT42200.08.2948621
CGGAGAT125350.08.2379531
CGCGAAT19600.08.2152991
GGGCAAT51450.08.1642711
GGGAGAT89900.08.0988861
GGGAAAC62050.08.0670711
GCCAGAT88000.08.0350821
CGGACAT48250.07.98025851