FastQCFastQC Report
Wed 5 Oct 2016
HKNHCBGXY_n01_timecourse_shoot_15n2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKNHCBGXY_n01_timecourse_shoot_15n2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15036964
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAAT23650.010.5083151
GGAAAAC55350.09.9285971
CGGGCAT38200.09.8045191
CCGGGAT63850.09.6484521
CGGGTAT16400.09.6044951
CTCGGAT36500.09.4939781
GCCGAAT28650.09.4074511
CGCAAAT27150.09.2825771
GGAAAAT78850.09.1891331
GGGAGAT78550.09.1351051
GGGAAAT51600.08.9542681
CCGGAAT28600.08.9343451
GCCAGAT71300.08.5912111
CGGGAAT24050.08.5870271
GGGACAT41350.08.5497171
CGGCTAT21500.08.4658551
GCCGATT41550.08.424321
GCCAAAT62750.08.3672631
CTCGAAT29950.08.2978851
CGGGACT19050.08.2684371
Produced by FastQC (version 0.11.2)