FastQCFastQC Report
Wed 5 Oct 2016
HKNHCBGXY_n01_timecourse_shoot_15n1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKNHCBGXY_n01_timecourse_shoot_15n1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15969807
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGAAT28500.010.5629571
CGGGGAT58150.010.2336641
CGGGCAT41900.09.440531
GGAAAAT89800.09.3165151
GGGAAAT56800.09.1826891
CCGGGAT66750.09.177381
CGCGTAT11850.09.1574651
GGGACAT45500.09.0013241
GCCGGAT59350.08.9061121
GTCGAAT30100.08.8385091
GCCAGAT78950.08.8233371
CGGCAAT51550.08.6239651
GGGAGAT83600.08.5419251
GCGGAAT23200.08.2986341
GGGGAAT44750.08.2917221
GGAAAAC62250.08.0975761
CGCGAAT16900.08.0781111
GCCAAAT68400.08.0348081
CCGGAAT28600.07.9557151
GGGATAT39400.07.9072541
Produced by FastQC (version 0.11.2)