FastQCFastQC Report
Wed 5 Oct 2016
HKNHCBGXY_n01_timecourse_shoot_15k3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKNHCBGXY_n01_timecourse_shoot_15k3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18449559
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAAAT67200.09.6882071
GGGAGAT95450.09.5711551
GGGATAT47350.09.2403781
GCCGAAT32250.09.1169441
GGGACAT51750.09.0634631
CCGGGAT71200.08.9964431
CGGGGAT66450.08.9547541
GGAAAAT105850.08.9283791
CGGGAAT29100.08.9009931
GCCAAAT80950.08.7776641
CGGAAAT30550.08.7076731
CGCGAAT20200.08.6639991
CGGGCAT46500.08.5812711
GGAAAAC75250.08.4657341
GGGGAAT51100.08.3567741
GTCGAAT34900.08.3243891
GGAAGAT172600.08.3146281
GGCAAAT81400.08.213131
CTCGAAT36750.08.1910731
GGCGAAT31800.08.1452481
Produced by FastQC (version 0.11.2)