FastQCFastQC Report
Wed 5 Oct 2016
HKNHCBGXY_n01_timecourse_shoot_15k2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKNHCBGXY_n01_timecourse_shoot_15k2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21820214
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAAT37600.010.8918481
GGAAAAT122350.09.7555981
CTCGGAT48550.09.0120651
GCGAAAT45200.08.8281551
GGGAAAT87150.08.5549151
GGAAGAT205200.08.5289621
GGCAAAT94350.08.4585611
CCGGGAT78950.08.423741
GTCGAAT43100.08.3649521
GGGAGAT109000.08.3171921
GGGGAAT61350.08.215831
GGGACAT63100.08.2098621
GCCGAAT37650.08.1812791
CGGGAAT36100.08.0477491
GCGGGAT54700.07.9348351
GCGGAAT31500.07.77841231
GGGAAAC73550.07.662081
GCCAAAT92850.07.61505371
AATCGAA72650.07.61125475
AATCCAA161750.07.59447345
Produced by FastQC (version 0.11.2)