FastQCFastQC Report
Thu 28 Nov 2024
HKN5LAFX7_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKN5LAFX7_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11101831
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9771328.80153913349969No Hit
ATAACTGAAGATCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3244562.9225449387583002Illumina Single End PCR Primer 1 (100% over 38bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2314872.085124516847716Illumina Single End PCR Primer 1 (100% over 50bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGAGTAGATCTCGGTGGTCGCC1359881.2249150613083553Illumina Single End PCR Primer 1 (97% over 38bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCAGATCGGAAGAGCGTCGTGTA1109060.9989883650723921Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GTGACTGGAGTTCAGACGTGTGCTCAGATCGGAAGAGCGTCGTGTAGGGA641560.5778866567145545Illumina Single End PCR Primer 1 (100% over 25bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC522050.4702377472688964Illumina Single End PCR Primer 1 (97% over 38bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCAGATCGGAAGAGCGTCGTGTAG413470.372434060651797Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC389350.35070791475748464Illumina Single End PCR Primer 1 (100% over 38bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCGACTAGATCGGAAGAGCGTCG330440.2976445957428104Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GTGACTGGAGTTCAGACGTGTGCTCTAGATCGGAAGAGCGTCGTGTAGGG323430.29133032199823616Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GTGACTGGAGTTCAGACGTGTGCTAGATCGGAAGAGCGTCGTGTAGGGAA314190.28300737058598713Illumina Single End PCR Primer 1 (100% over 26bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCGAGATCGGAAGAGCGTCGTGT268120.24150971132599658Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GTGACTGGAGATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC256180.23075472865692154Illumina Single End PCR Primer 1 (97% over 40bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGTGTAGAACTCGGTGGTCGCC256040.2306286233324935Illumina Single End PCR Primer 1 (97% over 38bp)
ATAACTGAAGATCGTCCTGTAGGGAAAGAGAGTAGATCTCGGTGGTCGCC252830.22773720839382258No Hit
ATAACTGAAGATCGTCGTGTAGGGAAAGAGTGTAGATCACGGTGGTCGCC249950.22514304171987487Illumina Single End PCR Primer 1 (97% over 38bp)
GTGACTGGAGTTCAGACGTGTGCTCTTAGATCGGAAGAGCGTCGTGTAGG227060.20452482117589432Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGAGTAGATATCGGTGGTCGCC224150.20190363193242628Illumina Single End PCR Primer 1 (96% over 25bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGAGAAGATCTCGGTGGTCGCC209680.18886974590047353No Hit
ATAACTGAAGATCGTCGTGTAGGGAAAGAGAGTAGATCACGGTGGTCGCC166460.14993923074491045Illumina Single End PCR Primer 1 (96% over 26bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCGAAGATCGGAAGAGCGTCGTG166130.14964198248018729Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC161390.14537241649598162Illumina Single End PCR Primer 1 (100% over 40bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCGATTAGATCGGAAGAGCGTCG159320.14350785919908166Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GTGACTGGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT155410.13998591763827067Illumina Single End PCR Primer 1 (100% over 39bp)
GTGACTGGAGTTCAGACGTGTGCAGATCGGAAGAGCGTCGTGTAGGGAAA139810.12593418148772037Illumina Single End PCR Primer 1 (100% over 27bp)
ATAACTGAAGATCGTCCTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC137410.12377237592609723Illumina Single End PCR Primer 1 (97% over 38bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGAGTAGAACTCGGTGGTCGCC134970.12157454027178038Illumina Single End PCR Primer 1 (95% over 24bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATC134660.12129530705340408Illumina Single End PCR Primer 1 (97% over 38bp)
ATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC122810.1106213920928899Illumina Single End PCR Primer 1 (98% over 50bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGTGAAGATCTCGGTGGTCGCC118920.10711746557842576Illumina Single End PCR Primer 1 (97% over 38bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCGATAGATCGGAAGAGCGTCGT113710.1024245460050689Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ATAACTGAAGATCGTCCTGTAGGGAAAGAGAGAAGATCTCGGTGGTCGCC113400.10214531278669256No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)