FastQCFastQC Report
Thu 28 Nov 2024
HKN5LAFX7_n02_jz7340_2_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKN5LAFX7_n02_jz7340_2_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7005754
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3316654.734179932666777Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1052781.5027361794319354No Hit
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC240320.343032313152874Illumina Single End PCR Primer 1 (100% over 38bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC206950.2954000383113652Illumina Single End PCR Primer 1 (100% over 40bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC149000.21268231799175366Illumina Single End PCR Primer 1 (100% over 38bp)
GTGACTGGAGTTCAGACGTGTGCTCAGATCGGAAGAGCGTCGTGTAGGGA102120.14576589472025425Illumina Single End PCR Primer 1 (100% over 25bp)
GTGACTGGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT99280.14171208409544497Illumina Single End PCR Primer 1 (100% over 39bp)
GTGACTGGAGTTCAGACGTGTGCTAGATCGGAAGAGCGTCGTGTAGGGAA73890.10547044615040722Illumina Single End PCR Primer 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)