FastQCFastQC Report
Thu 28 Nov 2024
HKN5LAFX7_n02_jz7340_2_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKN5LAFX7_n02_jz7340_2_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7327125
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC105593114.411259532217615Illumina Single End PCR Primer 1 (100% over 50bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1670742.2802122251224044Illumina Single End PCR Primer 1 (100% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1520802.075575345036423No Hit
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC954541.3027483494549363Illumina Single End PCR Primer 1 (100% over 40bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC776651.0599655390074552Illumina Single End PCR Primer 1 (100% over 38bp)
GTGACTGGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT561490.7663169387720286Illumina Single End PCR Primer 1 (100% over 39bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCAGATCGGAAGAGCGTCGTGTA427410.5833256563795486Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGAGTAGATCTCGGTGGTCGCC344070.46958390910486714Illumina Single End PCR Primer 1 (97% over 38bp)
GTGACTGGAGTTCAGACGTGTGCTCAGATCGGAAGAGCGTCGTGTAGGGA244790.33408738079396766Illumina Single End PCR Primer 1 (100% over 25bp)
GTGACTGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG221490.302287732227852Illumina Single End PCR Primer 1 (100% over 41bp)
GTGACTGGAGTTCAGACGTGTGCTAGATCGGAAGAGCGTCGTGTAGGGAA151770.2071344490506167Illumina Single End PCR Primer 1 (100% over 26bp)
GTGACTGGAGTTCAGACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG149600.20417285087943768Illumina Single End PCR Primer 1 (100% over 32bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCAGATCGGAAGAGCGTCGTGTAG137420.18754968695088456Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC135760.1852841325895218Illumina Single End PCR Primer 1 (97% over 38bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATC120200.16404797243120595Illumina Single End PCR Primer 1 (97% over 38bp)
GTGACTGGAGTTCAGACGTGTGCTCTAGATCGGAAGAGCGTCGTGTAGGG112740.15386662572291315Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCGACTAGATCGGAAGAGCGTCG112460.1534844840234062Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCGAGATCGGAAGAGCGTCGTGT86770.11842298309364177Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GTGACTGGAGTTCAGACGTGTGCAGATCGGAAGAGCGTCGTGTAGGGAAA84760.11567975160789531Illumina Single End PCR Primer 1 (100% over 27bp)
GTGACTGGAGTTCAGACGTGTGCTCTTAGATCGGAAGAGCGTCGTGTAGG81360.11103945954245356Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GTGACTGGAGTTCAGACGTGTGAGATCGGAAGAGCGTCGTGTAGGGAAAG80740.11019328863640242Illumina Single End PCR Primer 1 (100% over 28bp)
GTGACTGGAGTTCAGACGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG77230.10540286947472575Illumina Single End PCR Primer 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)