FastQCFastQC Report
Thu 28 Nov 2024
HKN5LAFX7_n02_jz7340_2_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKN5LAFX7_n02_jz7340_2_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8094864
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC170551721.06912481790923Illumina Single End PCR Primer 1 (100% over 50bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC2805803.466148412128975Illumina Single End PCR Primer 1 (100% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2421822.9917982562770664No Hit
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC1599801.976314858408986Illumina Single End PCR Primer 1 (100% over 40bp)
GTGACTGGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT919151.1354730604491934Illumina Single End PCR Primer 1 (100% over 39bp)
GTGACTGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG369940.4570058249280038Illumina Single End PCR Primer 1 (100% over 41bp)
GTGACTGGAGTTCAGACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG251990.3112961502503316Illumina Single End PCR Primer 1 (100% over 32bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATC157590.1946789964599776Illumina Single End PCR Primer 1 (97% over 38bp)
GTGACTGGAGTTCAGACGTGTGAGATCGGAAGAGCGTCGTGTAGGGAAAG131150.16201631058903523Illumina Single End PCR Primer 1 (100% over 28bp)
GTGACTGGAGTTCAGACGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG125240.15471538496508402Illumina Single End PCR Primer 1 (100% over 30bp)
AGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGCC104280.12882242369976815Illumina Single End PCR Primer 1 (98% over 50bp)
GGACTGGAGTTCAGACGTGTGCTCTTCCGACTAGATCGGAAGAGCGTCGT82790.10227472629558694Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)