FastQCFastQC Report
Thu 28 Nov 2024
HKN5LAFX7_n02_jz7340_2_09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKN5LAFX7_n02_jz7340_2_09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5862322
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC103549817.6636152023038Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1884143.2139824458636017No Hit
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC1579112.693659611328071Illumina Single End PCR Primer 1 (100% over 38bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC938991.6017373320673958Illumina Single End PCR Primer 1 (100% over 40bp)
GTGACTGGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT502780.8576465093524375Illumina Single End PCR Primer 1 (100% over 39bp)
GTGACTGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG194130.33114864724250903Illumina Single End PCR Primer 1 (100% over 41bp)
GTGACTGGAGTTCAGACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG160340.2735093705190537Illumina Single End PCR Primer 1 (100% over 32bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATC122020.20814278028398986Illumina Single End PCR Primer 1 (97% over 38bp)
AGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGCC103010.17571535647478934Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGTTCAGACGTGTGCTAGATCGGAAGAGCGTCGTGTAGGGAA95710.1632629528026608Illumina Single End PCR Primer 1 (100% over 26bp)
GTGACTGGAGTTCAGACGTGTGAGATCGGAAGAGCGTCGTGTAGGGAAAG92160.15720733183881747Illumina Single End PCR Primer 1 (100% over 28bp)
GTGACTGGAGTTCAGACGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG84990.14497668330057611Illumina Single End PCR Primer 1 (100% over 30bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGAGTAGATCTCGGTGGTCGCC81290.13866519102840139Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGTTCAGACGTGTGCTCAGATCGGAAGAGCGTCGTGTAGGGA78070.13317248694288714Illumina Single End PCR Primer 1 (100% over 25bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCACGGTGGTCGCC75860.1294026496668044Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAACTCGGTGGTCGCC75590.12894208131180784Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC71950.12273293756296566Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGAGATCTCGGTGGTCGCC65550.11181576174082557Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGTTCAGACGTGTGCAGATCGGAAGAGCGTCGTGTAGGGAAA64720.11039994050139176Illumina Single End PCR Primer 1 (100% over 27bp)
GGACTGGAGTTCAGACGTGTGCTCTTCCGACTAGATCGGAAGAGCGTCGT61220.10442960997365891Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGATTGTAGATCTCGGTGGTCGCC59740.101905013064789Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)