FastQCFastQC Report
Thu 28 Nov 2024
HKN5LAFX7_n02_jz7340_2_07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKN5LAFX7_n02_jz7340_2_07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12960805
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC161389212.45209691836271Illumina Single End PCR Primer 1 (100% over 50bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC2518251.9429734495658255Illumina Single End PCR Primer 1 (100% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2412011.8610032324381087No Hit
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC1596281.2316210297122747Illumina Single End PCR Primer 1 (100% over 40bp)
GTGACTGGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT929490.7171545285960247Illumina Single End PCR Primer 1 (100% over 39bp)
GTGACTGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG357670.27596279706391696Illumina Single End PCR Primer 1 (100% over 41bp)
GTGACTGGAGTTCAGACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG244780.18886172579558136Illumina Single End PCR Primer 1 (100% over 32bp)
GGACTGGAGTTCAGACGTGTGCTCTTCCGACTAGATCGGAAGAGCGTCGT183230.1413723916068485Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATAAAGGAATAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC139870.1079176794959881Illumina Single End PCR Primer 1 (100% over 40bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATC139570.10768621239190003Illumina Single End PCR Primer 1 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)