FastQCFastQC Report
Thu 28 Nov 2024
HKN5LAFX7_n02_jz7340_2_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKN5LAFX7_n02_jz7340_2_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7561680
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2348193.1053813438283555Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG596220.7884755768559367No Hit
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC359870.47591276012737915Illumina Single End PCR Primer 1 (100% over 38bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC174610.23091429417801337Illumina Single End PCR Primer 1 (100% over 40bp)
GTGACTGGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT99690.13183578252451836Illumina Single End PCR Primer 1 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)