FastQCFastQC Report
Thu 28 Nov 2024
HKN5LAFX7_n02_jz7340_2_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKN5LAFX7_n02_jz7340_2_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7022367
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC5364797.6395750891401715Illumina Single End PCR Primer 1 (100% over 50bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC885651.2611844410866022Illumina Single End PCR Primer 1 (100% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG799821.1389606951616171No Hit
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC452120.6438284982827016Illumina Single End PCR Primer 1 (100% over 40bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC318820.45400646249334453Illumina Single End PCR Primer 1 (100% over 38bp)
GTGACTGGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT221880.31596184021712337Illumina Single End PCR Primer 1 (100% over 39bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGAGTAGATCTCGGTGGTCGCC133410.1899786781294683Illumina Single End PCR Primer 1 (97% over 38bp)
GTGACTGGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG95650.1362076348330983Illumina Single End PCR Primer 1 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)