FastQCFastQC Report
Thu 28 Nov 2024
HKN5LAFX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKN5LAFX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11101831
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA113757810.24676019658379TruSeq Adapter, Index 14 (97% over 38bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCT1365091.2296079808817122TruSeq Adapter, Index 14 (97% over 34bp)
GAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAAC948470.8543365504302849TruSeq Adapter, Index 14 (96% over 30bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAT873500.7868071491990826TruSeq Adapter, Index 2 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTG597090.5378302011623127TruSeq Adapter, Index 14 (96% over 33bp)
AGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACT578570.521148268245121TruSeq Adapter, Index 14 (96% over 29bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCG575420.5183108984454907TruSeq Adapter, Index 14 (97% over 38bp)
AGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAA477880.43045151741185755TruSeq Adapter, Index 14 (96% over 31bp)
AAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGA469520.42292122803887033TruSeq Adapter, Index 14 (96% over 32bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC383650.34557362654862966TruSeq Adapter, Index 14 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTC361720.3258201282292984TruSeq Adapter, Index 14 (97% over 35bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATC361280.3254237972096675TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCAGTCACAGATCGGAAGAGCACAC252290.22725080214245738Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTC194340.1750522053524324TruSeq Adapter, Index 14 (96% over 28bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC189700.17087271459996103TruSeq Adapter, Index 13 (97% over 35bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGT185180.1668013141255708TruSeq Adapter, Index 14 (97% over 36bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC171500.15447902242431902TruSeq Adapter, Index 13 (96% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG158620.1428773325769416TruSeq Adapter, Index 9 (100% over 49bp)
AGATGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC136590.123033759025876TruSeq Adapter, Index 14 (97% over 34bp)
TCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGG135480.1220339239536253TruSeq Adapter, Index 14 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACG132020.11891732093561865TruSeq Adapter, Index 14 (97% over 37bp)
AGACGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC124650.11227877635680097TruSeq Adapter, Index 13 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC114940.10353247135540074TruSeq Adapter, Index 14 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)